DA-9601 inhibits activation of the human mast cell line HMC-1 through inhibition of NF-κB

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S. Lee1, H.-H. Park1, H.-Y. Son1, J.-H. Ha1, M.-G. Lee1, T.-Y. Oh2, D. H. Sohn3, T. C. Jeong4, S. H. Lee4, J.-K. Son4, S. G. Lee5, C.-D Jun5 and S.-H. Kim1, 6 Contact Information

(1)  Department of Pharmacology, Kyungpook National University Medical School, Daegu, Korea
(2)  Dong-A Pharmaceutical Research Institute, Yongin, Korea
(3)  Department of Pharmacy, Medicinal Resources Research Institute, Wonkwang University, Iksan, Korea
(4)  College of Pharmacy, Yeungnam University, Gyongsan, Korea
(5)  Department of Life Science, Gwangju Institutive of Science and Technology, Gwangju, Korea
(6)  Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, 700–422, Republic of Korea

Received: 15 March 2006  Accepted: 12 September 2006  Published online: 8 November 2006

Abstract  Mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 with immune regulatory properties. The formulated ethanol extract of Artemisia asiatica Nakai (DA-9601) has been reported to have antioxidative and anti-inflammatory activities. In this report, we investigated the effect of DA-9601 on the expression of pro-inflammatory cytokines by the activated human mast cell line HMC-1 and studied its possible mechanisms of action. DA-9601 dose-dependently decreased the gene expression and production of TNF-α, IL-1β, and IL-6 on phorbol 12-myristate 13-acetate (PMA)- and calcium ionophore A23187-stimulated HMC-1 cells. In addition, DA-9601 attenuated PMA- and A23187-induced activation of NF-κB as indicated by inhibition of degradation of IκBα, nuclear translocation of NF-κB, NF-κB/DNA binding, and NF-κB-dependent gene reporter assay. Our in vitro studies provide evidence that DA-9601 might contribute to the treatment of mast cell-derived allergic inflammatory diseases.

Keywords  allergic inflammation - DA-9601 - mast cell - NF-κB - pro-inflammatory cytokine