Journal of Ethnopharmacology
Volume 88, Issues 2-3, October 2003, Pages 269-273
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doi:10.1016/S0378-8741(03)00235-6 | How to Cite or Link Using DOI
Copyright © 2003 Elsevier Ireland Ltd. All rights reserved. Cited By in Scopus (14)
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Inhibitory effects of DA-9601 on ethanol-induced gastrohemorrhagic lesions and gastric xanthine oxidase activity in rats
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Keun Huha, Tae Hyup Kwonb, Uk Sup Shinc, Won Bae Kimd, Byoung Ok Ahnd, Tae Young Ohd and Jung-Ae Kim, , a
a College of Pharmacy, Yeungnam University, Gyongsan 712-749, South Korea
b Department of Pharmacy, Gyongbuk National University Hospital, Daegu 700-721, South Korea
c Department of Pharmacy, Dongguk University Hospital, Gyongju 780-350, South Korea
d Research Institute, Dong-A Pharmaceutical Co. Ltd., Yongin 449-905, South Korea
Received 17 March 2003; revised 28 May 2003; accepted 4 July 2003. ; Available online 13 August 2003.
Abstract The exposure of gastric mucosa to ethanol produces pathological changes such as inflammatory process, hemorrhagic erosions, even acute ulcers. The gastric mucosal lesions accompanied by a significant decrease of gastric blood flow and increase of reactive oxygen species (ROS) implicate a role of xanthine oxidase in ethanol-induced gastric hemorrhagic erosions. DA-9601, a novel antipeptic formulation of extracts of Artemisia asiatica Nakai, was studied for its inhibitory effect on gastric xanthine oxidase activity and type conversion of the enzyme that has a profound role in free radical generation. Intubation of absolute ethanol (4 g/kg) significantly induced gastrohemorrhagic lesions and lipid peroxidation in the rat stomach. Oral administration of DA-9601 at 40 mg/kg body weight significantly reduced ethanol-induced gastric mucosal hemorrhagic lesions and lipid peroxidation, which was proportional to the inhibitory effect of DA-9601 on alcohol-induced xanthine oxidase-type conversion and enzyme activity. The results suggest that alcohol-induced gastric mucosal damage may be, in part, due to the increased activity of xanthine oxidase and type conversion rate of the enzyme and that the preventive effect of DA-9601 on gastrohemorrhagic lesions would result from its inhibitory action against xanthine oxidase and oxidative stress in alcohol-treated rats.
Author Keywords: DA-9601; Artemisia asiatica; Alcohol; Gastrohemorrhagic lesion; Lipid peroxidation; Xanthine oxidase
Article Outline
1. Introduction
2. Materials and methods
2.1. Animals and treatments
2.2. Gastroantral hemorrhagic lesion measurement
2.3. Enzyme assay
2.4. Determination of lipid peroxidation
2.5. Data analysis
3. Results
3.1. Effect of DA-9601 on the ethanol-induced gastrohemorrhagic lesions in rats
3.2. Effect of DA-9601 on the gastric lipid peroxidation in ethanol-treated rats
3.3. Effect of DA-9601 on the activity and type conversion of gastric xanthine oxidase in ethanol-treated rats
4. Discussion
Acknowledgements
References
Fig. 1. Effects of DA-9601 on gastric lipid peroxidation in ethanol-treated rats. The assay procedure is described in Section 2. The other conditions are same as in Table 1. C, control; DA, DA-9601 (40 mg/kg, p.o.); E, ethanol (4 g/kg, p.o.); DA + E, co-treated with DA-9601 (40 mg/kg, p.o.) and ethanol (4 g/kg, p.o.). *P<0.05 compared to control. #P<0.05 compared to ethanol-treated group.
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Table 1. Effects of DA-9601 on ethanol-induced gastric mucosal hemorrhagic lesions in rats
Rats were administered absolute ethanol (4 g/kg, p.o.) after the treatment with the DA-9601 (40 mg/kg, p.o.) for 3 days and sacrificed 2 h after the ethanol treatment. *P<0.05 compared to control. #P<0.05 compared to ethanol-treated group.
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Table 2. Effects of DA-9601 on gastric xanthine oxidase activity in ethanol-treated rats
Rats were administered absolute ethanol (4 g/kg, p.o.) after the treatment with the DA-9601 (40 mg/kg, p.o.) for 3 days and sacrificed 2 h after the ethanol treatment. *P<0.05 compared to control. #P<0.05 compared to ethanol-treated group.
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